The characterization of fluidic microsystems serves to ensure functionality and reproducibility in biological, pharmaceutical, and diagnostic applications. Structural, fluidic, and functional parameters are captured using advanced measurement and analysis methods in order to describe flow behavior, mechanical properties, and the interaction between the biological sample and the microsystem. Depending on the application, standardized test procedures or specifically adapted protocols are employed, which, in addition to functional parameters, also evaluate stability and long-term performance, thereby enabling the deployment and optimization of robust, versatile microsystems.